Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  5

Year :  2012

Pages :   633-636

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Method for Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet Dosage Form



Address:   Rima N. Shah, Deesha B. Gandhi and Mehul M. Patel*
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis , Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, At and Post- Changa, Ta- Petlad, Dist.- Anand. 388421, Gujarat, India.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, selective, accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for the Simultaneous estimation of Amlodipine Besylate and Indapamide in combined tablet dosage form.Chromatographic separation achieved isocratically on a C18 Column utilizing a mobile phase of Acetonitrile:Water contains 0.2% of Triethyl amine and adjusting the pH to 4.0 with orthophosphoric acid (70:30, v/v, pH 4.0) at a flow rate of 0.8 ml/min with UV detection at 238nm. The retention time for Amlodipine Besylate and Indapamide was found to be 3.27 and 4.7 min respectively. Linearity was obtained in the concentration range of 20-120 µg/ml of Amlodipine Besylate and 6-36 µg/ml of Indapamide with a correlation coefficient of 0.9999 and 0.9996 respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation.This study aimed at developing and validating an HPLC method, being simple, accurate, robust and selective, and the proposed method can be used for the estimation of these drugs in combined dosage forms.
KEYWORDS:
Simultaneous Estimation; RP-HPLC; Amlodipine Besylate; Indapamide.
Cite:
Rima N. Shah, Deesha B. Gandhi, Mehul M. Patel. RP-HPLC Method for Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet Dosage Form. Asian J. Research Chem. 5(5): May 2012; Page 633-636.
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