Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  5

Year :  2012

Pages :   576-581

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Spectrophotometric estimation of Nebivolol hydrochloride and HPTLC method for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in bulk drug and in pharmaceutical formulation



Address:   Savita S Yadav, Janhavi R Rao*
Department of Pharmaceutical Chemistry, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy Erandwane, Pune -411038, India
*Corresponding Author
DOI No:

ABSTRACT:
A rapid assay procedure have been developed for the determination of Nebivolol hydrochloride based on application of oxidant to the spectrophotometry and high performance thin layer chromatography (HPTLC) have been developed for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in a bulk drug and pharmaceutical formulation. In spectrophotometric method, Nebivolol Hydrochloride is treated with a known excess of cerium (IV) sulphate and the residual oxidant is determined by treating with a fixed amount of indigo carmine, and measuring the absorbance at 610 nm and Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60GF-254, with chloroform: toluene: methanol: ammonia (5:3:2:0.1, v/v/v/v) as mobile phase. Detection was performed densitometrically at 278 nm. The RF of Nebivolol and Hydrochlorothiazide were 0.30 and 0.42, respectively. In spectrophotometry, Beer’s law is obeyed over concentration range of 2- 10 µg/mL. The results of the proposed procedures were validated statistically. The proposed methods were simple, accurate, and rapid and can therefore be used for routine analysis of both drugs in quality control laboratories.
KEYWORDS:
Nebivolol hydrochloride, Hydrochlorothiazide, Spectrophotometry, HPTLC, Cerium (IV) sulphate.
Cite:
Savita S Yadav, Janhavi R Rao. Spectrophotometric estimation of Nebivolol hydrochloride and HPTLC method for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in bulk drug and in pharmaceutical formulation. Asian J. Research Chem. 5(5): May 2012; Page 576-581.
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