Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  5

Year :  2012

Pages :   600-605

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Isolation of Apigenin From Trigonella foenum Graecum Seed Extract Using Column Chromatograpy on Lab Scale



Address:   Ms. Shivani Watak, Dr. Mrs. Swati S. Patil
Prin. K.M. Kundnani College of Pharmacy, Cuffe Parade, Colaba, Mumbai.
*Corresponding Author
DOI No:

ABSTRACT:
Methanolic extract of Trigonella foenum graecum seeds contains flavonoids such as rutin and Apigenin as major constituent. Column chromatographic technique was used as attempt on lab scale for separation of Apigenin by using butanol: ethyl acetate (1: 1) as mobile phase. The compound isolated from column was evaluated by performing HPTLC, UV, IR and HPLC with standard Apigenin. This method showed an excellent performance in separating the flavonoid Apigenin
KEYWORDS:
Column chromatography, Trigonella foenum graecum, Apigenin.
Cite:
Shivani Watak, Swati S. Patil. Isolation of Apigenin From Trigonella foenum Graecum Seed Extract Using Column Chromatograpy on Lab Scale. Asian J. Research Chem. 5(5): May 2012; Page 600-605.
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