Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  8

Year :  2012

Pages :   966-971

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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High Performance Liquid Chromatography Method for the Determination of Dextropropoxyphene in Hair and Nail



Address:   Cijo John1*, Priyankar Ghosh2, K.M. Varshney2, S.K. Shukla1, S.Satyanarayana3
1Central Forensic Science Laboratory, Govt. of India, Chandigarh, India-160 036
2Central Forensic Science Laboratory, Govt. of India, Hyderabad, India- 500 013
3Department of Chemistry, Osmania University, Hyderabad, India- 500 007
*Corresponding Author
DOI No:

ABSTRACT:
Dextropropoxyphene, a narcotic analgesic is one of the highly abused drugs in recent times. An analytical methodology was developed for the detection and quantitation of dextropropoxyphene in hair and nail samples collected from drug addicts. The method involves the decontamination of hair and nail using 2ml each of 0.1% SDS (sodium dodecyl sulphate), water and methanol sequentially under ultrasonication of 3 minutes duration and followed by digestion with methanol. The digested samples were undergone Solid Phase Extraction (SPE) using normal silica stationary phase with acetonitrile as the eluent and the extracts were taken for HPLC analysis. The separation was achieved on a Phenomenex Gemini C18 HPLC reversed-phase column (150mmx4.6mmx5µ) with diode array detection with UV range 200- 370 nm and quantification carried out at 217nm. The calibration plot for the determination of dextropropoxyphene is based on linear regression analysis, y= 13.584x + 52.885 with a linear regression coefficient of r2= 0.9990. The limit of detection of dextropropoxyphene was estimated as 0.5µg/ml and the limit of quantification was estimated as 2µg/ml. The amount of dextropropoxyphene detected in the hair and nail was found to be in the range of 0.0433- 0.0383 µg/mg and 0.1828-0.0696 µg/mg respectively. The presence of dextropropoxyphene is confirmed by injection of the fraction collected from HPLC to a GC-MS system. The method is rapid and reliable for the qualitative and quantitative analysis of dextropropoxyphene in hairs and nails and it can be used by law enforcement laboratories for routine analysis.
KEYWORDS:
Hair and nail analysis, Dextropropoxyphene, Drug abuse, HPLC, GC-MS.
Cite:
Cijo John, Priyankar Ghosh, K.M. Varshney, S.K. Shukla, S.Satyanarayana. High Performance Liquid Chromatography Method for the Determination of Dextropropoxyphene in Hair and Nail. Asian J. Research Chem. 5(8): August, 2012; Page 966-971.
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