Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  9

Year :  2012

Pages :   1190-1194

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Separation and quantification of Phytohormone production from Methylotrophs (MSF 34) using High performance liquid chromatography



Address:   Anitha Naren* and Sagaai Vilma P.
Shri AMM Murugappa Chettiar Research Centre, Taramani, Chennai-600 113, India
*Corresponding Author
DOI No:

ABSTRACT:
In this study, intracellular and extracellular phytohormone production was investigated in the methylotroph MSF34. The qualitative and quantitative analysis of these hormones was done using specific bioassays and high performance liquid chromatography (HPLC) technique respectively. Our findings showed that the MSF34 bacteria used in this study synthesized the auxin Indole-3-acetic acid (IAA) and Abscisic acid (ABA). However IAA was found only in microbial spent medium whereas ABA was present in the cell extracts.
KEYWORDS:
Extracellular, HPLC, intracellular, methylotrophs, hormone.
Cite:
Anitha Naren, Sagaai Vilma P.. Separation and quantification of Phytohormone production from Methylotrophs (MSF 34) using High performance liquid chromatography. Asian J. Research Chem. 5(9): September, 2012; Page 1190-1194.
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