Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  9

Year :  2012

Pages :   1104-1107

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Estimation of Rizatriptan in bulk and pharmaceutical formulation



Address:   Jane Jacob*, Devin M. Dadhaniya, Patel Vikas, Jani Vishal M, Amit Bangad
Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, Paneer, Deralakatte-575018
*Corresponding Author
DOI No:

ABSTRACT:
Four simple, sensitive, accurate, precise, and economical methods were developed for the quantitative estimation of Rizatriptan in bulk drug and its pharmaceutical formulation. Method-1 was a HPLC method and the chromatographic separation was carried out on column of Zorbex-eclips XDB, C18, 150 x 4.6mm, 5µ. using a mobile phase composition of acetonitrile,methanol and phosphate buffer of pH 2.5 in ratio 45:30:25 v/v pumped at flow rate of 1 ml/min. The eluent was monitored at 278 nm. The method was linear over the range of 50-150 µg/ml. The method was statistically validated for precision, accuracy, robustness and recovery. Method-2 and Method-3 are colorimetric methods based on condensation reaction involving the formation of violet colored complex between Rizatriptan and vanillin in presence of conc. Sulphuric acid which showed a linearity range 3-18 µg/ml at 558nm and formation of red colored complex between Rizatriptan and chromogenic agent 4-aminophenazone in presence of potassium ferricyanide which obeyed Beer’s law in the concentration range of 20-100 µg/ml and showed absorption maxima of 531nm. Method-4 was a simple UV method developed and validated for the estimation of Rizatriptan in methanol. The linearity range for Rizatriptan was obtained as 10-50 µg/ml and its wavelength of detection was 278nm. The results of analysis for all the four methods have been validated statistically according to ICH guidelines.
KEYWORDS:
Rizatriptan, HPLC, colorimetric methods.
Cite:
Jane Jacob, Devin M. Dadhaniya, Patel Vikas, Jani Vishal M, Amit Bangad. Estimation of Rizatriptan in bulk and pharmaceutical formulation. Asian J. Research Chem. 5(9): September, 2012; Page 1104-1107.
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