Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  9

Year :  2012

Pages :   1118-1122

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A RP-HPLC method for the simultaneous estimation of paracetamol and nabumetone in API and in tablet dosage form



Address:   C.K.Oza1*, Ranju Nijhawan1, M. K. Pandya2, A. J. Vyas3, A. I. Patel3
1.Xylopia, Ahmadabad, India.
2.R.K University, Rajkot, India.
3.B. K. Mody Govt. Pharmacy College, Rajkot, India.
*Corresponding Author
DOI No:

ABSTRACT:
A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of Nabumetone and Paracetamol. The separation was achieved by octadecyl column (C18) and Acetonitrile: water (75:25 v/v) at pH 6.5 using ortho phosphoric acid as eluent, at a flow rate of 1.0 ml/min. UV detection was carried out at 239.0 nm. The retention time of NABU and PCM was found to be 4.9 and 2.7 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Nabumetone and Paracetamol were in the range of 2-20 µg/ml for both drugs. The correlation coefficients were better than 0.999 and 0.999 for NABU and PCM respectively. The lower limit of detection (LOD) for NABU and PCM were found to be 0.27 and 0.31, respectively. The lower limit of quantification (LOQ) for NABU and PCM were found to be 0.81 and 0.93 respectively. The % recovery obtained for Nabumetone and Paracetamol were 99.66% to 101.00 % and 99.68% to 100.66 % respectively. The developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Nabumetone and Paracetamol in tablets.
KEYWORDS:
Reverse phase high performance liquid chromatography (RP-HPLC), Nabumetone, Paracetamol, ortho phosphoric acid.
Cite:
C.K.Oza, Ranju Nijhawan, M. K. Pandya, A. J. Vyas, A. I. Patel. A RP-HPLC method for the simultaneous estimation of paracetamol and nabumetone in API and in tablet dosage form. Asian J. Research Chem. 5(9): September, 2012; Page 1118-1122.
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