Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  1

Year :  2013

Pages :   58-63

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Reverse Phase High Performance Liquid Chromatographic Method for the Simultaneous Estimation of Amoxicillin Trihydrate and Tinidazole in the Tablet Dosage Form



Address:   Bojaraju V., Sireesha D., Mahesh Nasare, V.V.L.N. Prasad and PrakashV. Diwan
Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301
*Corresponding Author
DOI No:

ABSTRACT:
A simple, rapid and selective RP -HPLC method has been developed for determination of Amoxicillin trihydrate and Tinidazole in the pharmaceutical dosage form. The chromatographic separation was achieved with reverse phase phenomenex ® (Luna 5µ C18(2) 100A (250 × 4.60 mm) Column in isocratic mode and mobile phase containing 25mM Potassium dihydrogen phosphate (adjusted to pH 4.5 by Ortho-phosphoric acid) and Acetonitrile in the ratio of 40:60 % v/v. The flow rate was 1mL min-1 and effluent was monitored at 238 nm. Retention time was found to be 2.053 min for Amoxicillin trihydrate and 2.944 min for Tinidazole , respectively. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 5-50 µg/ml for both Amoxicillin trihydrate and Tinidazole , respectively. Percentage recoveries obtained for Amoxicillin trihydrate and Tinidazole were 100.87% and 99.52% respectively. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of Amoxicillin trihydrate and Tinidazole in pharmaceutical dosage forms.
KEYWORDS:
Amoxicillin trihydrate, Tinidazole , RP-HPLC Method, Validation, Simultaneous estimation.
Cite:
Bojaraju V., Sireesha D., Mahesh Nasare, V.V.L.N. Prasad, PrakashV. Diwan. Reverse Phase High Performance Liquid Chromatographic Method for the Simultaneous Estimation of Amoxicillin Trihydrate and Tinidazole in the Tablet Dosage Form. Asian J. Research Chem. 6(1): January 2013; Page 58-63.
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