Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  10

Year :  2013

Pages :   926-931

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Optimization and Establishment of Validated stability indicating RP-HPLC Method for Simultaneous Estimation of Mefenamic acid and Paracetamol from Tablet Dosage Form



Address:   Sudesh D. Shambharkar*, Purnima D. Hamrapurkar
Department of Pharmaceutical Analysis, Prin K.M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaokar Marg, Cuffe Parade, Colaba, Mumbai-400 005, India
*Corresponding Author
DOI No:

ABSTRACT:
A simple, selective and precise gradient HPLC method has been developed for simultaneous estimation of Mefenamic acid and Paracetamol combination from pharmaceutical dosage form. The separation was accomplished on an HiQSil reversed phase C18 column, 250 mm ×4.6mm I.D., 5µm column using two mobile phases i.e. A as methanol: 50mM potassium dihydrogen orthophosphate (pH = 7.5)(20:80 v/v) and B as methanol in a gradient elution mode. The flow rate was 1mL/min. The eluents were monitored with a UV detector set at 245 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness and high sensitivity. Detection and quantification limits were established at 0.02 µg/ml and 0.05 µg/ml respectively. A stability indicating HPLC method has been established for analysis of Mefenamic acid and Paracetamol combination. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis, and thermal decomposition.
KEYWORDS:
Mefenamic acid, Paracetamol, Gradient elution, stability indicating method, HPLC.
Cite:
Sudesh D. Shambharkar, Purnima D. Hamrapurkar. Optimization and Establishment of Validated stability indicating RP-HPLC Method for Simultaneous Estimation of Mefenamic acid and Paracetamol from Tablet Dosage Form. Asian J. Research Chem. 6(10): October 2013; Page 926-931.
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