Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  11

Year :  2013

Pages :   1003-1006

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Method Development and Validation for the Determination of Ibandronic Acid in Solid Dosage Form by RP-HPLC



Address:   Rajesh Balkrishna Tawade, Dr. Abha Sham Dhomane
K.V. Pendharkar College, Dombivali, Mumbai, Maharashtra, India.
*Corresponding Author
DOI No:

ABSTRACT:
A high-performance liquid chromatographic method has been developed for the analysis of Ibandronic acid10 in solid dosage form1. The mobile phase consisting of EDTA and Hexane sulphonic acid buffer by adjusting pH (pH 2.5) and acetonitrile in ratio of (95:5 % v/v) was delivered at the flow rate of 1.0 mL/min and UV detection was carried out at 195 nm1. The separation was achieved using C18 reverse-phase column (250 X 4.6 mm I.D., particle size 5┬Ám) 7 . The method was linear over the concentration range for Ibandronic acid. The analytical recovery obtained was 99.88%. The validation of method carried out as per ICH guidelines 4. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing Ibandronic acid in solid dosage form and can be employed for bioequivalence study in future for the same formulations.
KEYWORDS:
RP-HPLC, Ibandronic Acid, Validation.
Cite:
Rajesh Balkrishna Tawade, Abha Sham Dhomane. Method Development and Validation for the Determination of Ibandronic Acid in Solid Dosage Form by RP-HPLC. Asian J. Research Chem. 6(11): November 2013; Page 1003-1006.
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