Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  2

Year :  2013

Pages :   144-147

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Studies on the Effect of Organophosphate, Dichlorvos on the Esterases of seeds of an Underutilized Legume, Mucuna pruriens



Address:   Kempohalli S. Chandrashekharaiah
Department of Biotechnology, PESIT, BSK III Stage, Bangalore-560085, Karnataka, India
*Corresponding Author
DOI No:

ABSTRACT:
The esterases were isolated from the seeds of eight varieties/accessions of an underutilized legume, Mucuna and studied. The seeds of all the varieties of Mucuna exhibited esterase. Among the different varieties analyzed for esterase activity, Mucuna pruriens exhibited highest ester hydrolyzing activity (4.7µmoles/min) and specific activity (0.018) while Mucuna cochinensis showed the lowest esterase activity (0.309µmoles/min) and specific activity (0.0017). Germination of Mucuna pruriens has been carried out and the change in esterases was monitored. Effect of organophosphate such as dichlorvos on the activities of esterases was analyzed. The esterases are completely inhibited by dichlorvos. The Mucuna seed esterases are found to be carboxylesterases.
KEYWORDS:
Esterases, Mucuna seeds, organophosphates, dichlorvos, inhibition.
Cite:
Kempohalli S. Chandrashekharaiah. Studies on the Effect of Organophosphate, Dichlorvos on the Esterases of seeds of an Underutilized Legume, Mucuna pruriens. Asian J. Research Chem. 6(2): February 2013; Page 144-147.
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