Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  2

Year :  2013

Pages :   148-154

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Separation evaluation of selected organophosphorus fungicides by NP-TLC and RP-HPTLC



Address:   Purigali M Nagaraju1,3, Praveen U Sanganalmath2**, Kempaiah Kemparaju3, Bannur M Mohan4
1Regional Forensic Science Laboratory, Davanagere, India.
2Regional Forensic Science Laboratory, Gulbarga, India.
3Department of Studies in Biochemistry, Mysore University, Mysore, India.
4Forensic Science Laboratory, Bangalore, India.
**Corresponding Author
DOI No:

ABSTRACT:
Poisoning by organophosphorus fungicides (OPFs) is although uncommon; recently, a few cases of deaths owing to the ingestion of OPFs were reported. Literatures on chromatographic separation of these fungicides are minimal. The evaluation of the efficacies of separation is of primary interest when it is contemplated from a forensic matrix. Hence, a study was undertaken to evaluate the efficacies of separation of three OPFs, namely ditalimfos D, edifenfos E, and tolclofos-methyl TM by NP-TLC on silica gel 60F254 and RP- HPTLC on silica gel 60RP-18 WF254. The relationship between the mobile phase composition (n-hexane-acetone for NP-TLC, and methanol-water for RP-HPTLC) and RF values affirmed that with the increase in the acetone content in case of NP-TLC and decrease of the water content in RP-HPTLC resulted in the increase in RF values for the three OPFs. The three OPFs are completely separated from each other with ?RF=0.04, except for the pair of compounds E-D in NP-TLC with mobile phase composition 10:0, and for the pair of compounds E-TM in case of RP-HPTLC with mobile phase compositions of 10:0, 7:3, 6.5:3.5 and 6:4. Under the chromatographic conditions used, D and TM were found to have higher RM values in normal phase and reverse phase, respectively. Ditalimfos was therefore adsorbed strongly in NP-TLC and tolclofos-methyl in RP-HPTLC. The peak resolution (RS) values in NP-TLC were greater than 1.5 for the pair of compounds E-D, TM-E, and TM-D in solvent composition ratio of 9:1, 8.5:1.5, 8:2, and 7.5:2.5. RS values in RP-HPTLC were also higher for all the pair compounds, except for E-TM. The study revealed that the system of NP-TLC with mobile phase n-hexane-acetone (9:1) provided the optimum conditions for the separation of D, E, and TM as compared to RP- HPTLC system. Complete separation of E from TM was not achieved in RP-HPTLC.
KEYWORDS:
NP-TLC, RP-HPTLC, Mobile phase composition, Peak resolution.
Cite:
Purigali M Nagaraju, Praveen U Sanganalmath, Kempaiah Kemparaju, Bannur M Mohan. Separation evaluation of selected organophosphorus fungicides by NP-TLC and RP-HPTLC. Asian J. Research Chem. 6(2): February 2013; Page 148-154.
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