Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  2

Year :  2013

Pages :   172-176

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Estimation of Pioglitazone Hydrochloride in Bulk Drug and Pharmaceutical Dosage Forms by Hydrotropy Technique and Oxidative-Coupling Reaction



Address:   Ramakrishna K.*, Rebecca Shiffali D., Pani Kumar A.D.
Department of Pharmaceutical Analysis and Quality Assurance, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad-500 090 Andhra Pradesh, India
*Corresponding Author
DOI No:

ABSTRACT:
Two simple, selective, rapid, accurate, precise and cost-effective spectrophotometric methods for the determination of Pioglitazone Hydrochloride in bulk drug and in tablets have been developed and validated. In the first method (method A), hydrotropic solution of citric acid (0.1M) was employed as solubilizing agent to solubilize pioglitazone hydrochloride (poorly water soluble drug) fine powder and its tablet dosage form for spectrophotometric determination in UV region (hydrotropic technique). The maximum absorbance of pioglitazone hydrochloride in 0.1M citric acid solution by hydrotropic technique was found to be at 269 nm. The second method (method B) is based on the reaction of 3- methylbenzothiazolin-2-one hydrazone (MBTH) with pioglitazone hydrochloride in the presence of ferric chloride (oxidative-coupling reaction). The resulting green colored chromogen complex absorbs at ? (Lambda) max 667 nm. Beer’s law was obeyed over the ranges 4-34, 5-35 µg/mL for method A and method B respectively. The limit of detection (LOD), limit of quantification (LOQ) and sandell’s sensitivity values are reported. Both the methods were validated in accordance with ICH guidelines. The results of the analysis have been validated statistically. The methods have been successfully applied in the analysis of marketed formulations. No significant interference was observed from either hydrotropic agent or commonly used tablet additives with the assay procedure.
KEYWORDS:
Pioglitazone Hydrochloride, Estimation, Hydrotropy, Oxidative-Coupling.
Cite:
Ramakrishna K., Rebecca Shiffali D., Pani Kumar A.D.. Estimation of Pioglitazone Hydrochloride in Bulk Drug and Pharmaceutical Dosage Forms by Hydrotropy Technique and Oxidative-Coupling Reaction. Asian J. Research Chem. 6(2): February 2013; Page 172-176.
[View HTML]      [View PDF]



Visitor's No. :   120993