Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  3

Year :  2013

Pages :   236-240

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Spectrophotometric Estimation of Carbocisteine in Bulk and Pharmaceutical Dosage Form by First Order Derivative and Area Under Curve Methods



Address:   Rele Rajan V.*, Patil Sachin S.
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author
DOI No:

ABSTRACT:
A simple and precise first order derivative and area under curve [AUC] UV- spectrophotometric methods have been developed and validated for the estimation of carbocisteine in bulk and its tablet formulation. The standard and sample solutions of carbocisteine were prepared in 0.1 N HCl. Carbocisteine was estimated at 198.4 nm for the first order derivative UV-spectrophotometric method (A), while in area under curve (AUC) method (B) the zero order spectrum of carbocisteine was measured in between 197.4 nm to 210.2 nm. Beer’s law was obeyed in the concentration range of 10 to 140 µg / ml with coefficient of correlation value 0.9999 for first order derivative method. Similarly in AUC method, Beer’s law was obeyed in the concentration range of 10 to 140 µg / ml with coefficient of correlation value 0.9999. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation, which was within the range of 0.02560 % to 0.13375 % for the above two methods. The proposed methods were successfully applied for the determination of carbocisteine in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.
KEYWORDS:
Carbocisteine, Derivative spectroscopy, Area under curve method.
Cite:
Rele Rajan V., Patil Sachin S.. Spectrophotometric Estimation of Carbocisteine in Bulk and Pharmaceutical Dosage Form by First Order Derivative and Area Under Curve Methods. Asian J. Research Chem. 6(3): March 2013; Page 236-240.
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