Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  3

Year :  2013

Pages :   263-267

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Method development and validation for estimation of Felodipine in human plasma by LCMS/MS



Address:   Srinivas Reddy, Jibin V. Joseph, Aravind Kumar, Arindam Mukhopadhyay*, Saral Thangam
Norwich Clinical Services Pvt. Ltd., 147/F, 8th Main, 3rd Block, Koramangala, Bangalore– 560034
*Corresponding Author
DOI No:

ABSTRACT:
After extraction from plasma by liquid – liquid extraction method, Felodipine and FelodipineD5, (IS), were separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min. The analytes were detected in API 4000 Mass spectrometer in the positive atmospheric pressure chemical Ionization (APCI) mode with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 384.0 & daughter ion 338.0 (Felodipine), and m/z 389.1 & daughter 338.1 (Felodipine D5, IS). A linear calibration plot of Felodipine was achieved in the concentration ranges of 0.105 ng/ml to 20.000 ng/ml. Mean recovery was 69.4%.This method was fully validated for specificity, precision, accuracy, reproducibility and other criteria as per regulations.
KEYWORDS:
Felodipine, Liquid liquid extraction, LCMS/MS, Human plasma, Validation.
Cite:
Srinivas Reddy, Jibin V. Joseph, Aravind Kumar, Arindam Mukhopadhyay, Saral Thangam. Method development and validation for estimation of Felodipine in human plasma by LCMS/MS. Asian J. Research Chem. 6(3): March 2013; Page 263-267.
[View HTML]      [View PDF]



Visitor's No. :   106359