Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  3

Year :  2013

Pages :   204-208

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods



Address:   Preeti Tiwari1*, and Rakesh K. Patel2
1Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), India
2Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India
*Corresponding Author
DOI No:

ABSTRACT:
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin–Ciocalteu reagent and was found 0.1045 and 0.1041 %w/w gallic acid equivalent in Drakshasava-T and Drakshasava-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01264 and 0.01214 %w/w quercetin equivalent in Drakshasava-T and Drakshasava-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Drakshasava-T and Drakshasava-M. The antioxidant activity of Drakshasava-T and Drakshasava-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Drakshasava-T and Drakshasava-M showed significant scavenging of super-oxide radical and showed IC50 120.63 and 128.36 µg/ml respectively. Drakshasava-T and Drakshasava-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 212.50 and 220.15 µg/ml respectively. Marketed Drakshasava also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Drakshasava-T and Drakshasva-M can be a promising source of natural antioxidant.
KEYWORDS:
Total phenolics, flavonoids, antioxidant potential, Drakshasava.
Cite:
Preeti Tiwari, Rakesh K. Patel. Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods. Asian J. Research Chem. 6(3): March 2013; Page 204-208.
[View HTML]      [View PDF]



Visitor's No. :   106338