Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  4

Year :  2013

Pages :   341-344

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form



Address:   M.A.Haneef1, B.Rajkamal1, G. Tharun goud2, V. Mohan Goud2
1Ganga Pharmacy College, Nizamabad, A.P. India.
2University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar.
*Corresponding Author
DOI No:

ABSTRACT:
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
KEYWORDS:
Zidovudine, RP-HPLC, validation.
Cite:
M.A.Haneef, B.Rajkamal, G. Tharun goud, V. Mohan Goud. Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form. Asian J. Research Chem. 6(4): April 2013; Page 341-344.
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