Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  5

Year :  2013

Pages :   490-494

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin



Address:   G. Venkata Prasad, S. Sravani, B. Mohammed Ishaq*, M. Madhu, Sreenivasulu Munna,C. Gopinath
Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P. India.
*Corresponding Author
DOI No:

ABSTRACT:
To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5µg/ml to 40µg/ml (Method A) in Phosphate Buffer pH 2.0 and 5µg/ml to 50µg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 µg/mL and 2.85 µg/mL (Method A) and 2.357 µg/mL and 7.857 µg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.
KEYWORDS:
Cephalexin, spectroscopic method, Phosphate Buffer (pH 2.0), 0.1 N HCl.
Cite:
G. Venkata Prasad, S. Sravani, B. Mohammed Ishaq, M. Madhu, Sreenivasulu Munna, C. Gopinath. Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin. Asian J. Research Chem. 6(5): May 2013; Page 490-494.
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