Journal : Asian Journal of Research in Chemistry
Volume No. : 6
Issue No. : 7
Year : 2013
Pages : 613-617
ISSN Print : 0974-4169
ISSN Online : 0974-4150
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A. Gupta*, S. K. Das and A. K. Banik
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
*Corresponding Author
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DOI No:
ABSTRACT:
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB200 developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl2 in synthetic medium, 0.2 (M) CaCl2 for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.
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KEYWORDS:
Immobilization, L-lysine, calcium alginate bead, encapsulation, mutant.
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Cite:
A. Gupta, S. K. Das, A. K. Banik. Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200. Asian J. Research Chem. 6(7): July 2013; Page 613-617.
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