Journal :   Asian Journal of Research in Chemistry

Volume No. :   6

Issue No. :  9

Year :  2013

Pages :   855-858

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Reverse Phase High Performance Liquid Chromatographic Estimation of Atazanavir and Ritonavir in Pharmaceutical Dosage Form



Address:   Venkatesh J., M. Singaiah Chowdary*, Haritha D., Anuroop, Dr. V.V.L.N. Prasad and Anjani Prasad Reddy V.
Department of Pharmaceutical Analysis and Quality Assurance, School of Pharmacy, Anurag Group of Institutions, Venkatapur (V), Ghatkesar (M), Rangareddy (D) AP
*Corresponding Author
DOI No:

ABSTRACT:
A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of atazanavir and ritonavir in the combined pharmaceutical dosage form. The chromatographic separation for Atazanavir and Ritonavir were achieved with mobile phase containing mixed phosphate buffer (pH 4.0) and acetonitrile (45:55 % v/v), Symmetry C18 (4.6 x 100mm, 3.5?m, Make: ACE) at 50C and UV detection at 237 nm. The compounds were eluted in the isocratic mode at a flow rate of 0.9 ml min-1. The retention times of atazavir 4.29 ± 0.09 min and ritonavir at 5.018± 0.09 min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ etc. in accordance with ICH guidelines.
KEYWORDS:
Atazanavir; Ritonavir; RP-HPLC; Validation.
Cite:
Venkatesh J., M. Singaiah Chowdary, Haritha D., Anuroop, V.V.L.N. Prasad, Anjani Prasad Reddy V.. Reverse Phase High Performance Liquid Chromatographic Estimation of Atazanavir and Ritonavir in Pharmaceutical Dosage Form. Asian J. Research Chem. 6(9): September 2013; Page 855-858.
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