Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  10

Year :  2014

Pages :   870-876

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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LC-ESI-MS/MS method for Quantitation of Lovastatin in Rat Plasma and Liver Homogenate: Application to Pharmacokinetic Study

Address:   Sanjay Walode1*, Shailendra Gurav2, Avinash Kasture3
1Department of Pharmaceutical Chemistry, Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk), Lonavala, Pune, 410 401, India
2Department of Phytochemistry, Government College of Pharmacy, Karad - 415 110, India
3Department of Pharmaceutical Chemistry, University Department of Pharmaceutical Sciences, Nagpur – 440 010, India
*Corresponding Author

A sensitive method, using high-performance liquid chromatography-electrospray tandem mass spectrometry was developed for the determination of a hydrophilic liver-specific inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme-A reductase, lovastatin in rat plasma and rat liver homogenate. Simvastatin was used as an internal standard. In this method, samples were prepared in simple two-step liquid-liquid extraction with ethyl acetate. Isocratic chromatographic separation of reconstituted samples was done with mixture of 10 mM ammonium acetate buffer (pH 4): methanol (10:90,v/v) using Phenomenex ultra sphere guard (RP-18), 30x4.6mm, 5µm column. The detection was performed using API 4000 LC-MS/MS system (Applied Biosystem, MDS Sciex, USA) operated in ESI positive mode. The analysis was performed by multiple reactions monitoring mode. The calibration curve obtained were linear (r2=0.99) over the concentration range of 0.19-100 ng/mL for both plasma and liver homogenate. The LOD and LOQ for lovastatin were found to be 0.04 ng/mL and 0.19 ng/mL respectively. Lovastatin was found to be stable in plasma, liver homogenate and the reconstituted solution. The method is sensitive and reliable with a total run time of less than 2 min. The developed assay method was successfully applied for the determination of lovastatin in plasma sample collected in the pharmacokinetic study.
Lovastatin, simvastatin, LC-ESI-MS/MS, plasma, liver homogenate, pharmacokinetic.
Sanjay Walode, Shailendra Gurav, Avinash Kasture. LC-ESI-MS/MS method for Quantitation of Lovastatin in Rat Plasma and Liver Homogenate: Application to Pharmacokinetic Study. Asian J. Research Chem. 7(10): October- 2014; Page 870-876.
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