Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  11

Year :  2014

Pages :   119-924

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Kinetics of o-diphenol oxidase Immobilized on Agar- Abelmoschus esculentus Polymer



Address:   Anita S. Goswami Giri
Chemistry Research Laboratory, Department of Chemistry, B. N. Bandodkar College of Science, Chendani Bunder Road, Thane- 400 601. Maharashtra India
*Corresponding Author
DOI No:

ABSTRACT:
DOPA is a precursor of dopamine and acts as a neuro transmitter. Oxidation of it to dopaquinone is a fast reaction by o-diphenol oxidase. Discrepancies in this reaction are responsible for diverse type of disease and disorders. o-diphenol oxidase was purified on natural column and immobilized on agar- Abelmoschus esculentus polymer. Its immobilized o-diphenol oxidase activity showed Vmax =167┬Ámol/lit/min, Km= 2X10-3, Optimum PH = 6.8, Optimum temperature 800C, Ea = 160.45Kcal/mol and observed highest activity at 45min. behavior of encapsulated activity was compared with free o-diphenol oxidase.
KEYWORDS:
Enzyme-catalyzed reaction; Activation energy of PPO; onion leaves.
Cite:
Anita S. Goswami Giri. Kinetics of o-diphenol oxidase Immobilized on Agar- Abelmoschus esculentus Polymer. Asian J. Research Chem. 7(11): November, 2014; Page 119-924.
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