Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  11

Year :  2014

Pages :   933-939

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Quantitative Analysis of Polyamines in Paspalum scrobiculatum under Drought and Salt Stress



Address:   Siddappa N and Devaraj V R*
Department of Biochemistry, Central College, Bangalore University, Bangalore 560 001, India
*Corresponding Author
DOI No:

ABSTRACT:
Abiotic stresses are the leading cause of crop loss worldwide. Polyamine (PAs) levels are known to change in response to abiotic stresses. Levels of PAs were monitored after inducing drought stress in 9 days old seedlings by withholding water over 12 days, and inducing salt stress by treating with 150-450 mM NaCl over 72 h in half strength Hoagland medium. Quantitation of PAs in root/shoot tissues by Reverse phase HPLC indicated that Spd is the major polyamine metabolite in Paspalum scrobiculatum and greater elevation in Spd was noted in salt stressed seedlings in concentration and time dependent manner. Drought stress in Paspalum scrobiculatum caused steady increase in PAs, PUT, Spd, Spm, and Tspm up to 12 days. Compared to drought stressed seedlings, salt stressed shoot exhibited higher amounts of all the PAs, including Tspm. These results suggested efficient adaptation of the plant to gradual dehydration induced by drought, also, considerable ability to withstand rapid dehydration induced by NaCl up to 300 mM over 3 days by increased production of PAs.
KEYWORDS:
Kodo millet, drought, salinity, Polyamines.
Cite:
Siddappa N, Devaraj V R. Quantitative Analysis of Polyamines in Paspalum scrobiculatum under Drought and Salt Stress. Asian J. Research Chem. 7(11): November, 2014; Page 933-939.
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