Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  5

Year :  2014

Pages :   461-465

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Chemometrics-assisted Spectrophotometric Determination of Ciprofloxacin and Naphazoline in Eye Drops



Address:   Vu Dang Hoang
Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Vietnam
*Corresponding Author
DOI No:

ABSTRACT:
Multivariate calibration methods (Classical Least Squares, Inverse Least Squares and Principal Component Analysis) using the absorption spectra in the region of 225 – 265 nm were proposed for the resolution of strongly overlapping spectra of Ciprofloxacin and Naphazoline in binary mixtures. The quantitative predictive abilities of these methods were evaluated by the use of calibration matrix with nine synthetic mixtures of both compounds (24–36 mg/L of Ciprofloxacin and 4 – 6 mg/L of Naphazoline). They were also successfully applied to the simultaneous determination of Ciprofloxacin and Naphazoline in commercially available eye drops. Their performances were statistically compared by one-way ANOVA and Bartlett tests clearly indicating their similar accuracy and precision.
KEYWORDS:
UV spectra, Ciprofloxacin, Naphazoline, multivariate calibration, eye drops.
Cite:
Vu Dang Hoang. Chemometrics-assisted Spectrophotometric Determination of Ciprofloxacin and Naphazoline in Eye Drops. Asian J. Research Chem. 7(5): May 2014; Page 461-465.
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