Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  7

Year :  2014

Pages :   640-643

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Optimization of Enzymatic Synthesis of 3-O-β-D-Glucopyranoside Betulinic Acid by Novozyme-435



Address:   Hamisu Abdu1,3, Faujan B. H. Ahmad2, M. Basri1, Intan Safinar Ismail1, M.B. Abdul Rahman1
1Department of Chemistry, Faculty of Science, University Putra Malaysia, Malaysia.
2 Natural Product Laboratory, Faculty of Applied Sciences, Universiti Teknologi MARA, Malaysia.
3Hussaini Adamu Federal Polytechnic Kazaure, Jigawa State, Nigeria.
*Corresponding Author
DOI No:

ABSTRACT:
The enzymatic synthesis of 3-O-ß-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-ß-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30oC and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %.
KEYWORDS:
Enzymatic synthesis, 3-O-β-D-glucopyranoside betulinic acid, Novozyme-435 and optimization.
Cite:
Hamisu Abdu, Faujan B. H. Ahmad, M. Basri, Intan Safinar Ismail, M.B. Abdul Rahman. Optimization of Enzymatic Synthesis of 3-O-β-D-Glucopyranoside Betulinic Acid by Novozyme-435. Asian J. Research Chem. 7(7): July 2014; Page 640-643.
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