Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  8

Year :  2014

Pages :   717-721

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Zero Order and First Order Derivative Spectrophotometric Method for Determination of Rilpivirine Hydrochloride in Bulk Drug and Pharmaceutical Dosage Form



Address:   K. Vijaya Sri*, G. Vinay Jain, M. Madhuri
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad-500 014, Andhra Pradesh, India.
*Corresponding Author
DOI No:

ABSTRACT:
To develop a simple, fast and reliable derivative spectrophotometric methods for the estimation of rilpivirine hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in methanol and water (8:2). The quantitative determination of the drug was carried out using the zero order derivative values measured at 305nm and the first order derivative values measured at 271 nm (n=6). Linearity in the concentration range of 0.5–3.5 µg/ml and absorbance was measured at 305 nm and 271nm (r² = 0.999 and r² = 0.999) for zero order and first order derivative spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no much significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise, sensitive to assay of rilpivirine hydrochloride in pharmaceutical dosage form.
KEYWORDS:
Rilpivirine hydrochloride, Derivative spectrophotometric, Zero order derivative spectrum, First order derivative spectrum.
Cite:
K. Vijaya Sri, G. Vinay Jain, M. Madhuri. Zero Order and First Order Derivative Spectrophotometric Method for Determination of Rilpivirine Hydrochloride in Bulk Drug and Pharmaceutical Dosage Form. Asian J. Research Chem. 7(8): August 2014; Page 717-721.
[View HTML]      [View PDF]



Visitor's No. :   204339