Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  9

Year :  2014

Pages :   777-780

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Spectrophotometric Estimation of Ambroxal Hydrochloride and Guaiphenesin by Second Order Derivative Method in Combined Dosage Form



Address:   Rajan V. Rele*
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author
DOI No:

ABSTRACT:
The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. second order derivative method for the determination of ambroxal hydrochloride and guaiphenesin in combined dosage form i.e. tablets by using distilled water as a solvent. The method was further validated by ICH guidelines. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 220 nm and 234 nm were selected for the estimation of ambroxal hydrochloride and guaiphenesin respectively. The linearity of the proposed method was found in the concentration range of 1 to 10 µg /ml (r2= 0.9998) for ambroxal hydrochloride and 10 to 100 µg /ml (r2= 0.9999) for guaiphenesin respectively. The percentage mean recovery was found to be 99.928 % for ambroxal hydrochloride and 99.946 % for guaiphenesin respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.
KEYWORDS:
UV spectrophotometric estimation, second order derivative method, ambroxal hydrochloride, Guaiphenesin.
Cite:
Rajan V. Rele. Simultaneous Spectrophotometric Estimation of Ambroxal Hydrochloride and Guaiphenesin by Second Order Derivative Method in Combined Dosage Form. Asian J. Research Chem. 7(9): September 2014; Page 777-780.
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