Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  9

Year :  2014

Pages :   783-786

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Estimation of Eperisone Hydrochloride and Diclofenac Sodium in Pharmaceutical Dosage Form by RP- HPLC



Address:   K. Maniteja1*, Purvil Chovatia1, Jaya Naimisha1, Ch Pavani2, Prathima Patil2
1Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy and Research Centre, Brahmadevi (V), Nellore (Dist)-524346.
2Sushrut Institute of Pharmacy, Taddanpally (V), Pulkal (M), Medak (Dist)-502393.
*Corresponding Author
DOI No:

ABSTRACT:
A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in tablet dosage form. The separation was achieved by Nucleosil C18 (250 x 4.0 mm, 3 µm) column with Methanol: Acetonitrile: Triethylamine (50:50:2.0 ml v/v) as mobile phase, at a flow rate of 1.0 ml/min. Detection was carried out at 255 nm. Retention time of Diclofenac Sodium was found to be 2.10 minute and Retention time of Eperisone Hydrochloride was found to be 4.50 minute. The method has been validated for linearity, accuracy, precision and specificity. Linearity for Eperisone Hydrochloride and Diclofenac Sodium were in the range of 30-90µg/ml and 20-60µg/ml respectively. The percentage recoveries for Eperisone Hydrochloride and Diclofenac Sodium were found to be 101.14 ± 0.4413 and 99.27 ± 10.7322 respectively. The developed method was validated as per ICH guidelines. Developed method was found to be accurate, precise, specific and rapid for simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in pharmaceutical dosage form. The proposed method was successfully applied for the simultaneous estimation of both the drugs Eperisone Hydrochloride and Diclofenac Sodium in Capsule dosage form.
KEYWORDS:
Eperisone Hydrochloride, Diclofenac Sodium, RP-HPLC.
Cite:
K. Maniteja, Purvil Chovatia, Jaya Naimisha, Ch Pavani, Prathima Patil. Simultaneous Estimation of Eperisone Hydrochloride and Diclofenac Sodium in Pharmaceutical Dosage Form by RP- HPLC. Asian J. Research Chem. 7(9): September 2014; Page 783-786.
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