Journal :   Asian Journal of Research in Chemistry

Volume No. :   7

Issue No. :  9

Year :  2014

Pages :   787-794

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Stability Indicating HPLC Method for the Determination of Nicotine Hydrogen Tartrate



Address:   G.R. Shendarkar, P.S. Tarte*, M.H. Ghante, A.B. Roge
Nanded Pharmacy College, Shyam Nagar, Kasturba Matru Svea Bhavan, Shyam Nagar, Nanded-431605, Maharashtra, India
*Corresponding Author
DOI No:

ABSTRACT:
Nicotine hydrogen tartrate was subjected to different stress test conditions and solutions so obtained were further subjected to develop a stability-indicating high performance liquid chromatographic (HPLC) method. The drug was well separated from degradation products using a reversed-phase (Intersil C-18) column and a mobile phase comprising of methanol: buffer in ratio 25:75 v/v with a positive peak purity index. The detection limit and quantification limit were found to be 0.118 mg/ml and 0.354 mg/ml, respectively. The method was validated for precision, robustness, linearity and range. Results obtained after validation study, indicate that the proposed single method allowed analysis of nicotine hydrogen tartrate, in the presence of their degradation products formed under a different stress conditions.
KEYWORDS:
Nicotine hydrogen tartrate, HPLC, Validation, Stability, Degradation.
Cite:
G.R. Shendarkar, P.S. Tarte, M.H. Ghante, A.B. Roge. Stability Indicating HPLC Method for the Determination of Nicotine Hydrogen Tartrate. Asian J. Research Chem. 7(9): September 2014; Page 787-794.
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