Journal :   Asian Journal of Research in Chemistry

Volume No. :   8

Issue No. :  8

Year :  2015

Pages :   539-544

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Novel RP-HPLC Method for Simultaneous Estimation of Doxofylline and Sertraline in Bulk and Tablet Dosage Form



Address:   M.M. Eswarudu*, Y. Ravi Kumar and M. Chinna Eswaraiah
Department of Pharmaceutical Analysis, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda (Dt), 508206, Telangana State, India.
*Corresponding Author
DOI No: 10.5958/0974-4150.2015.00086.3

ABSTRACT:
The objective of this study was to develop a simple, rapid, accurate and precise isocratic stability indicating reversed-phase high-performance liquid chromatographic method for simultaneous estimation of Doxofylline and Sertraline in tablet dosage form. The separation method was carried out using Std XDB C18 column (150 x 4.6 mm; 5µ).The mobile phase used was a mixture of Acetonitrile and Potassium Di Hydrogen Phosphate buffer in the ratio of 45:55(v/v) delivered at an isocratic flow rate of 0.8mL/min. Column temperature was 300c and eluents were monitored at 231nm using Waters 2695 HPLC instrument equipped with the waters 2998 PDA detector and Empower-2 software for data collecting and processing. With the optimized method, the retention times of Doxofylline and Sertraline were found to be 2.06 min and 4.14 min respectively, with theoretical plate count and asymmetry as per the ICH limits. The method has shown a good linearity in the concentration range of 100-600 µg/ml for Doxofylline and 12.5-75µg/ml for Sertraline with regression coefficient (R2) of 0.999 and 0.997.The percentage assays were found to be 99.11 and 96.44 for Doxofylline and Sertraline. The method was found to be accurate (with percentage mean recoveries 99.71 for Doxofylline, and 99.10 for Sertraline), precise, robust, reliable, specific and stable. The proposed method was validated in accordance with ICH guidelines and hence this method can be successfully used for quantitative analysis of Doxofylline and Sertraline in tablet formulations.
KEYWORDS:
Doxofylline, Sertraline, RP- HPLC, Validation, Tablet dosage forms.
Cite:
M.M. Eswarudu, Y. Ravi Kumar, M. Chinna Eswaraiah. Novel RP-HPLC Method for Simultaneous Estimation of Doxofylline and Sertraline in Bulk and Tablet Dosage Form. Asian J. Research Chem. 8(8): August 2015; Page 539-544.
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