Journal :   Asian Journal of Research in Chemistry

Volume No. :   9

Issue No. :  12

Year :  2016

Pages :   629-632

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Liquid chromatography-mass spectrometric method for simultaneous estimation of apigenin and luteolin from Achillea millefolium Linn



Address:   Gomathy Subramanian1*, Subramania Nainar Meyyanathan2, Byran Gowramma, Dhanabal S. Palanisamy3
1Department of Pharmaceutical Chemistry, (A Constituent College of JSS University, Mysore), Ootacamund, The Nilgiris, Tamilnadu, India.
2Department of Pharmaceutical Analysis, (A Constituent College of JSS University, Mysore), Ootacamund, The Nilgiris, Tamilnadu, India.
3Principal, JSS College of Pharmacy, (A Constituent College of JSS University, Mysore), Ootacamund, The Nilgiris, Tamilnadu, India.
*Corresponding Author
DOI No: 10.5958/0974-4150.2016.00086.9

ABSTRACT:
To develop a rapid, specific, accurate and efficient liquid chromatography-mass spectrometry method and validate as per ICH guidelines for the simultaneous estimation of apigenin and luteolin from Achillea millefolium. The chromatographic separation was achieved by using C8 column, 150 x 4.6mm i.d., 5ยต Hibar Lichrospher, mobile phase containing 0.1% formic acid: acetonitrile (20:80 v/v). The flow rate was 0.4 ml/min. The retention time of apigenin and luteolin was found to be 8.48 min and 8.01 min respectively. Apigenin and luteolin exhibited linear in the range of 10-50 ng/ml. The method was validated for the parameters like system suitability, specificity, linearity, accuracy, precision, limit of detection and limit of quantification. The proposed method was successfully applied for the simultaneous estimation of both the constituents in Achillea millefolium and established a quantitative method for the simultaneous determination of apigenin and luteolin from Achillea millefolium.
KEYWORDS:
LC-MS method, Achillea millefolium, simultaneous estimation, flavonoids, apigenin, luteolin.
Cite:
Gomathy Subramanian, Subramania Nainar Meyyanathan, Byran Gowramma, Dhanabal S. Palanisamy. Liquid chromatography-mass spectrometric method for simultaneous estimation of apigenin and luteolin from Achillea millefolium Linn. Asian J. Research Chem. 2016; 9(12): 629-632.
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