Journal :   Asian Journal of Research in Chemistry

Volume No. :   10

Issue No. :  3

Year :  2017

Pages :   349-353

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Evaluation of Residues of Chlorpyrifos and its metabolite in green apples



Address:   Tentu Nageswara Rao1*, Botsa Parvatamma2, Karri Apparao1, Jakka Mastan1
1Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.
2Department of Organic Chemistry, Gayathri PG College, Vizianagaram, Andhra Pradesh, India.
*Corresponding Author
DOI No: 10.5958/0974-4150.2017.00059.1

ABSTRACT:
A simple and inexpensive method was developed using solid-phase extraction, together with gas chromatographic method and high performance liquid chromatographic method for determination of chlorpyrifos and its metabolite O,O-diethyl triphosphate residues in green apples. The evaluated parameters include the extracts by silica gel packed column using methanol and water solvent mixture (90:10) and ethyl acetate solvents. The method was validated using green apple fruit samples spiked with chlorpyrifos and its metabolite (O,O-diethyl triphosphate) at different fortification levels (0.01 and 0.1 mg/kg). Average recoveries (using each concentration six replicates) of range 86-95%. The calibration solutions concentration in the range 0.01-5.0 mg/L and limit of detection (LOD) and limit of quantification (LOQ) were 0.003mg/kg and 0.01mg/kg respectively. Finally the green apple fruit residue samples were analyzed by GC and HPLC.
KEYWORDS:
HPLC, GC, Silica gel, chlorpyrifos, O,O-diethyl triphosphate and green apples.
Cite:
Tentu Nageswara Rao, Botsa Parvatamma, Karri Apparao, Jakka Mastan. Evaluation of Residues of Chlorpyrifos and its metabolite in green apples. Asian J. Research Chem. 2017; 10(3):349-353.
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