Journal :   Asian Journal of Research in Chemistry

Volume No. :   11

Issue No. :  1

Year :  2018

Pages :   111-116

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A New Simple RP – HPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Tablet Dosage Form



Address:   B. Balaswami1*, Dr. P. Venkata Ramana1, B. Subba Rao2, P. Sanjeeva3
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003 A.P, India.
*Corresponding Author
DOI No: 10.5958/0974-4150.2018.00023.8

ABSTRACT:
A simple, accurate, economical, rapid, selective, reverse phase high performance liquid chromatography (RP-HPLC) was developed for simultaneous estimation of Meropenem and Vaborbactam in its tablet dosage form. The separation was carried out using a mobile phase of buffer and acetonitrile in the ratio of 50:50 pumped at a flow rate of 1 ml/min along with 250nm as a UV detection wavelength. The stationary phase used was column Kromasil 250 x 4.6 mm, 5m. Meropenem and Vaborbactam were eluted at a retention time of Meropenem 2.47min and Vaborbactam 3.31 min. The method was developed and validated as per ICH guidelines by considering the parameters such as precision, accuracy, linearity, specificity, robustness and degradation studies. The developed RP-HPLC method can be used for routine analysis of Meropenem and Vaborbactam in combinational dosage form.
KEYWORDS:
RP-HPLC method development, Validation, Meropenem, Vaborbactam.
Cite:
B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva. A New Simple RP – HPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Tablet Dosage Form. Asian J. Research Chem. 2018; 11(1):111-116.
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