Journal :   Asian Journal of Research in Chemistry

Volume No. :   11

Issue No. :  2

Year :  2018

Pages :   275-278

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Reverse Phase High Performance Liquid Chromatography for Method Development Validation of Omeprazole in Bulk and Pharmaceutical Dosage Form



Address:   Rajan V. Rele, Prathamesh P. Tiwatane
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai , 400 016.
*Corresponding Author
DOI No: 10.5958/0974-4150.2018.00051.2

ABSTRACT:
The validation of omeprazole from combined dosage form i.e. tablets was described by high performance liquid chromatography method with separation of drugs on BDS Hypersil C18 (150 x 4.6 mm i.d.) and 5 µ particle size. A mixture of buffer and acetonitrile (65:35 % (v/v)) was constituted as mobile phase. The chromatograms were studied at 230 nm as wavelength. The mobile phase was also used as a diluent. A validated of method was studied for linear regression, accuracy, method as well as system precision. The robustness study was done for change in wavelength, mobile phase composition and flow rate as per ICH guidelines. The method has been successfully used to analyze omeprazole from dosage form i.e. tablets and drotaverine from combined dosage form i.e. tablets.
KEYWORDS:
Omeprazole, tri-ethyl amine, ortho phosphoric acid acetonitile.
Cite:
Rajan V. Rele, Prathamesh P. Tiwatane. Reverse Phase High Performance Liquid Chromatography for Method Development Validation of Omeprazole in Bulk and Pharmaceutical Dosage Form. Asian J. Research Chem. 2018; 11(2):275-278.
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