Journal :   Asian Journal of Research in Chemistry

Volume No. :   12

Issue No. :  3

Year :  2019

Pages :   187-190

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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UV Spectrophotometric Estimation of Metformin hydrochloride by Area under Curve Methods in Bulk and Pharmaceutical Dosage Form



Address:   Rajan V. Rele*
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author
DOI No: 10.5958/0974-4150.2019.00035.X

ABSTRACT:
Simple and precise UV spectrophotometric method by area under curve [AUC] - have been developed and validated for the estimation of metformin hydrochloride in bulk and its tablet formulation. The standard and sample solutions of metformin hydrochloride were prepared in distilled water. Metformin hydrochloride was estimated UV spectroscopy by area under curve (AUC) method, the zero order spectrum of metformin hydrochloride was measured in between 228 nm to 236 nm. Beer’s law was obeyed in the concentration range of 1 to 14 µg/ml with coefficient of correlation value 0.9998. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 0.3625% respectively. The proposed method was successfully applied for the determination of metformin hydrochloride in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed method is simple, easy to apply, low-cost and require relatively inexpensive instruments.
KEYWORDS:
Metformin hydrochloride, UV spectroscopy, Area under curve method.
Cite:
Rajan V. Rele. UV Spectrophotometric Estimation of Metformin hydrochloride by Area under Curve Methods in Bulk and Pharmaceutical Dosage Form. Asian J. Research Chem. 2019; 12(3):187-190.
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