Journal :   Asian Journal of Research in Chemistry

Volume No. :   12

Issue No. :  6

Year :  2019

Pages :   295-302

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Förster Resonance Energy Transfer Investigation between PPO and Coumarin 151 Dyes and Docking studies with Prolyl-tRNA Synthetase Conformers



Address:   Mohmedyasin F. Mansuri1, Shravan B. Rathod2*
1Department of Microbiology, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India
2Department of Chemistry, Smt. S. M. Panchal Science College (H. N. G. University, Patan), Mahiyal Road, Talod-383215, North-Gujarat, India
*Corresponding Author
DOI No: 10.5958/0974-4150.2019.00054.3

ABSTRACT:
The Fluorescence Resonance Energy Transfer (FRET) between 2,5-diphenyl-1,3-oxazole (PPO) and 7-amino-4-(trifluoromethyl) chromen-2-one (Coumarin 151) dyes was investigated. In these studies, PPO behaves as a donor and Coumarin 151 as an acceptor. All the measurements were carried out in DMSO solvent. The quantum yield of the donor was found 0.48 using tryptophan as a standard at 280 nm excitation wavelength. Energy transfer was confirmed by excitation spectra, steady-state fluorescence quenching, and Time-correlated single photon counting measurements. Measured Förster distance (R0) and FRET efficiency (E) of the system are observed 33 Å and 0.24 respectively. Binding energies of the donor, acceptor and their derivatives with two conformers of Prolyl-tRNA synthetase (PDB: 4TWA, 4YDQ) are found different in magnitudes by docking studies.
KEYWORDS:
FRET, Energy transfer, Excitation, Binding energy, Docking.
Cite:
Mohmedyasin F. Mansuri, Shravan B. Rathod. Förster Resonance Energy Transfer Investigation between PPO and Coumarin 151 Dyes and Docking studies with Prolyl-tRNA Synthetase Conformers. Asian J. Research Chem. 2019; 12(6):295-302.
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