Journal :   Asian Journal of Research in Chemistry

Volume No. :   13

Issue No. :  1

Year :  2020

Pages :   1-6

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Optimization of Extraction conditions of the Polyphenols, Flavonoids and the Antioxidant activity of the plant Ammosperma cinereum (Brassicaceae) through the Response Surface Methodology (RSM)



Address:   Bouaziz-Benzid Amina1, Hammoudi Roukia1, Hadj Amahammed Mahfoud1, Tlili Ahlem1, Bouaziz sabrina2, Bekka Chahrazed3, Mesrouk Houria1
1Biogeochemistry Laboratory of Desert Environments. Univ. Kasdi Merbah Ouargla, Ouargla 30000, Algeria
2Ecosystems Protective Laboratory of Arid and Semi-arid Zones. Univ. Kasdi Merbah Ouargla, Ouargla 30000, Algeria
3Valuation and Promotion of Saharan Resources Laboratory. Univ. Kasdi Merbah Ouargla, Ouargla 30000, Algeria
*Corresponding Author
DOI No: 10.5958/0974-4150.2020.00001.2

ABSTRACT:
This work focuses on the optimization of the extraction conditions, to extract the maximum polyphenols (TPC), flavonoids (TF) and for optimal antioxidant activity by the response method to (RSM) areas, from the aerial part of Ammosperma cinereum. Independent variables considered are: the methanol percentage (X1: 60-100%), temperature (X2: 20-60°C) and the extraction time (X3: 20 -100 min). Results of ANOVA showed that the proportion of methanol, the temperature and duration of extraction affected TPC and TF, while the antioxidant activity was affected only by the methanol rate. The experimental data were well adjusted by polynomial models of the second order with R2 = 0.986, 0.962 and 0.985 for TPC, TF and I% respectively (p <0.005). In addition, there's a close relationship between the predicted and experimental values. The optimum conditions for the highest performance of TPC (34.16 ± 0.24 mg EAG / g of extract), TF (29.37 ± 1.09 mg ER / g of extract) with 64.82% (± 0.26) of the antioxidant activity were obtained X1 = 93.1%, X2 = 36.9 ° C and for X3 = 66.8 min.
KEYWORDS:
Ammosperma cinereum, RSM, Box Behnken, TPC, TF, antioxidant activity
Cite:
Bouaziz-Benzid Amina, Hammoudi Roukia, Hadj Amahammed Mahfoud, Tlili Ahlem, Bouaziz Sabrina, Bekka Chahrazed, Mesrouk Houria. Optimization of Extraction conditions of the Polyphenols, Flavonoids and the Antioxidant activity of the plant Ammosperma cinereum (Brassicaceae) through the Response Surface Methodology (RSM). Asian J. Research Chem. 2020; 13(1):01-06.
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