Journal :   Asian Journal of Research in Chemistry

Volume No. :   13

Issue No. :  1

Year :  2020

Pages :   48-51

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Derivative UV Spectrophotometric Method for Validation of Emtricitabine in Bulk and Pharmaceutical Dosage Form



Address:   Rajan V. Rele *, Prathamesh P. Tiwatane
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016
*Corresponding Author
DOI No: 10.5958/0974-4150.2020.00011.5

ABSTRACT:
Simple and precise derivative UV spectrophotometric method i.e. first order derivative method have been developed and validated for the estimation of emtricitabine in bulk and its tablet formulation. The standard and sample solutions of emtricitabine were prepared in distilled water. Emtricitabine was estimated by first order at wavelength 269 nm respectively. Beer’s law was obeyed in the concentration range of 1 to 20 µg / ml with coefficient of correlation value 0.9999. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation was 0.7852 %for the method respectively. The proposed method was successfully applied for the determination of emtricitabine in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed method is simple, easy to apply, low-cost and require relatively inexpensive instruments.
KEYWORDS:
Emtricitabine, First order derivative UV spectroscopy.
Cite:
Rajan V. Rele, Prathamesh P. Tiwatane. Derivative UV Spectrophotometric Method for Validation of Emtricitabine in Bulk and Pharmaceutical Dosage Form; Asian J. Research Chem. 2020; 13(1): 48-51.
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