Journal :   Asian Journal of Research in Chemistry

Volume No. :   13

Issue No. :  2

Year :  2020

Pages :   141-145

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Spectrophotometric estimation of Amoxicillin and Cloxacillin in their Pharmaceutical Dosage Form



Address:   Moni Ghosh, Suddhasattya Dey, Avijit Chatterjee, Saptarshy Sarkar, Sourav Khawas
Dr. B. C. Roy College of Pharmacy and Health Sciences, Durgapur-713206, West Bengal, India
*Corresponding Author
DOI No: 10.5958/0974-4150.2020.00028.0

ABSTRACT:
A new, simple, rapid and novel spectrophotometric method has been developed for estimation of Amoxicillin (AMX) and Cloxacillin (CLX) in bulk and combined pharmaceutical formulations using absorbance correction method (ACM). ? max of AMX was found at 209nm and CLX was at 249nm respectively. This method involved measurement of absorbance at two wavelengths of AMX and CLX i.e., 209 nm for AMX and 249nm for CLX. The combination is also estimated by ACM. It showed linearity of both the drugs at one wave lengths. Calibration curve was constructed at 209nm for AMX and 249nm for CLX respectively. Beer’s law obeyed in concentration range of 10- 50µg/mL for AMX and 10-50µg/mL for CLX respectively by the method. These methods were validated for precision, reproducibility, linearity and accuracy as per ICH guidelines. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate, cost effective and also sensitive and specific. It involves neither heating nor use of any organic solvent for separation of the combination. This study thus exploits the possibility for determining Pharmacokinetic data of the combined formulation which may be required in pre-clinical and clinical study in near future.
KEYWORDS:
Amoxicillin, Cloxacillin, UV Spectroscopy, Validation.
Cite:
Moni Ghosh, Suddhasattya Dey, Avijit Chatterjee, Saptarshy Sarkar, Sourav Khawas. Simultaneous Spectrophotometric estimation of Amoxicillin and Cloxacillin in their Pharmaceutical Dosage Form.
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