Journal :   Asian Journal of Research in Chemistry

Volume No. :   13

Issue No. :  2

Year :  2020

Pages :   113-116

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Estimation of Paroxetine hydrochloride and Clonazepam in Tablet Dosage form by HPLC



Address:   Sonali S. Gadge1*, Madhuri D. Game2, Vikrant L. Salode1, Aashish D. Nakod1, Mahesh S. Gadge1
1P R Patil Institute of Pharmacy, Talegaon (SP), Dist- Wardha (MS) India- 442202.
2Vidybharati College of Pharmacy, C.K. Naidu road, Camp, Amravati (MS) India-444602
*Corresponding Author
DOI No: 10.5958/0974-4150.2020.00023.1

ABSTRACT:
The main aim of this study was focuses on the simultaneous estimation of Paroxetine hydrochloride and Clonazepam in tablet dosage form by HPLC. The important objective was to develop and validate the method for accuracy. A simple, new and correct high performance liquid action technique was developed for coinciding determination of Clonazepam and Paroxetine in tablet dosage form by employing a C18 Intersil 250 × 4.6 millimeter column and mobile section consisting of Acetonitrile: 0.1 % OPA (65: 35 v/v). The analysis was performed at a flow rate of 0.8 ml/min. The method was linear over the concentration range 75 – 375 µg /ml and 3 – 15 µg/ml for Paroxetine and Clonazepam respectively with limits of detection of 2.99, 0.1464 and limit of quantification of 9.0791, 0.4438 for Paroxetine and Clonazepam respectively. The average % recovery was found to be 101.61 ±1.31 and 100.03± 1.15 for Paroxetine and Clonazepam respectively. The retention times (tR) of Paroxetine and Clonazepam were found to be 4.7667 and 7.6667 respectively. The correlation coefficient for both the drugs were found to be 0.999. The method was validated in terms of accuracy, precision, ruggedness, linearity and range. The developed method is specific, rapid, specific, cheap which is suitable for the estimation of Paroxetine hydrochloride and Clonazepam in tablet dosage form.
KEYWORDS:
Paroxetine hydrochloride, Clonazepam, High performance liquid chromatography, method validation, chromatogram.
Cite:
Sonali S. Gadge, Madhuri D. Game, Vikrant L. Salode, Aashish D. Nakod, Mahesh S. Gadge. Simultaneous Estimation of Paroxetine hydrochloride and Clonazepam in Tablet Dosage form by HPLC. Asian J. Research Chem. 2020; 13(2):113-116.
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