Journal :   Asian Journal of Research in Chemistry

Volume No. :   13

Issue No. :  2

Year :  2020

Pages :   117-122

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Spectrophotometric Estimation of Rifampicin, Isoniazid and Pyrazinamide in their Pharmaceutical Dosage Form



Address:   Sourav Khawas, Sampurna Parui, Suddhasattya Dey, Sudip Kr. Mondal, Saptarshy Sarkar
Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur-713206, West Bengal, India
*Corresponding Author
DOI No: 10.5958/0974-4150.2020.00024.3

ABSTRACT:
A simple, accurate, precise, reproducible and economical UV spectrophotometric method was developed and validated for the estimation of Isoniazid (INH), Rifampicin (RIF) and Pyrazinamide (PYZ) in their bulk and pharmaceutical dosage form. First order derivative spectrophotometric method was used. Wavelength for Isoniazid is 721nm (zero crossing point of Rifampicin and Pyrazinamide), for Rifampicin is 322nm (zero crossing point of Isoniazid and Pyrazinamide) and for Pyrazinamide it is 256nm (zero crossing point of Isoniazid and Rifampicin). Beer Lambert’s law was obeyed within the concentration range of 0.75-3.75µg/ml for Isoniazid, 1.5-7.5µg/ml for Rifampicin and 4-12µg/ ml for Pyrazinamide. The proposed method was been validated statistically as per the ICH guidelines for linearity, accuracy, precision, specificity, LOD and LOQ. The method developed and validated successfully for the quantitative analysis of Isoniazid, Rifampicin and Pyrazinamide in bulk and dosage form.
KEYWORDS:
Isoniazid, Rifampicin, Pyrazinamide, validation, UV Spectroscopy.
Cite:
Sourav Khawas, Sampurna Parui, Suddhasattya Dey, Sudip Kr. Mondal, Saptarshy Sarkar. Simultaneous Spectrophotometric Estimation of Rifampicin, Isoniazid and Pyrazinamide in their Pharmaceutical Dosage Form. Asian J. Research Chem. 2020; 13(2):117-122.
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