Analytical Parameters of Isolated Constituents of Root’s of Syzygium cuminii (L) skeel


Nikhat F.*, Satyanarayana D., Purohit M.G., Bharat, Moid A.

Department of Pharmaceutical Chemistry, Luqman College of Pharmacy, Gulbarga-585201

*Corresponding Author E-mail:



Syzygium cuminii (L) skeel commonly known as ‘jamun’ is widely used in Indian folk medicine for the treatment of diabetes Mellitus. The present study an attempt has been made to produce a complete analytical and chemical profile of the isolated constituents of root’s of syzygium cuminii(L) skeel . The pharmacological and pharmaceutical work is going on in our laboratory.


KEYWORDS: Syzygium cuminii (L) skeel, Constituents, diabetes




Diabetes mellitus was known to ancient Indian physicians as ‘madumeha’. Many herbal products including several metals and minerals have been described for the care of diabetes mellitus in ancient literature1. Ayurveda is an ancient Indian form of medicine, which deals with parts of our subcontinent. The jamun belongs to the family Myrtaceae and is called Syzigium cumini2. A literature survey shows that there is no work has been done on roots. In our preliminary study we have published article3 on the matter of extraction, isolation and interpretation of compounds obtained. Here the extension of work is reported with the hope that which may help research in this field and also to the common people.


Spectrometric analytical reports are not fond in the literature for its qualitative estimation for the preparation of pharmaceutical dosage form. Hence it was thought worthwhile to develop spectrometric methods for the same. This paper describe the simple sensitive, economical visible spectrometric method for quantitative estimation of ScRex-6b ( Myristin gentobioes)4.



All the spectral absorbance measurements were made on Shimadzu-1700 double beam UV-Visible spectrophotometer with 2cm Quartz cell was used for all spectral measurements. All the chemicals used were of analytical grade. All the solutions were freshly prepared with methanol. The stock solution was freshly prepared by dissolving 25mg of the pure isolated sample (roots Syzygium cuminii (L) skeel) in 25 ml of methanol to give concentration of 1mg/ml(1000µg/ml). From standard solution take 5ml of solution in 50ml of methanol to produce 50 µg/ml solution aliquot of 2,4,6,8,10, in a volumetric flask (10ml) and make up the volume to 10 ml  to produce the concentration as 10,,20,30,40,and 50 µg/ml. The spectrometric absorbance were measured at 312nm against methanol as the blank and the calibration graph was plotted.



Six different concentrations 2-10 µg/ml of ScRex-6b (Myristin gentobioes) which exhibiting the λmax 312. The absorbance spectrum and calibration curve where prepared for linear studies the response were measured as peak area and plotted against concentration. The stability of the compound were studied and it was found to be stable for 3.0 h the Optical characteristics such as Absorption maxima (nm), Beears law limit (µg/ml), Molar absorptivity (L/mol-1 cm-1), Sandells sensitivity (µg/ml 0.001 absorbance unit) and Correlation coefficient (r) were calculated of the method as shown in the Table the result where found to be satisfactory. The proposed method is simple, sensitive, accurate and precise and can be applied successfully for routine estimation in pharmaceutical dosage form.


Optical characteristics of ScRex-6b ( Myristin gentobioes) by UV-Spectrophotometric method



λmax (nm)


Beears law limit (µg/ml)


Molar absorptivity (L/mol-1 cm-1)


Regreation equation (Y=mx+c)


Slop (b)


Intersept (a)


Standard deviation


Relative Standard deviation


Correlation coefficient (r)


Sandells sensitivity (µg/ml 0.001 absorbance unit)


% Range of error


Confidence limit with 0.05 level


Confidence limit with 0.01 level


Limit of detection


Limit of Quantification


*Y=bc+a, where Y is the absorbance and c is concentration of screx-6b µg/ml; **For six replicates




The authors are grateful to the management of Luqman College of pharmacy Gulbarga for providing the necessary facility to carry out the work. The authors also thanks to the HOD of department of pharmaceutics Dr. N. G. Raghavendra Rao for providing the valuable suggestion



1.       Nadkarni, A.K., 1992. Indian Materia Medica, vol. 1. Popular Prakashan, Bombay, p. 157.

2.       Samba-Murthy, A.V.S.S., Subrahmanyam, N.S., 1989. Fruits. In: Johry, V.S. (Ed.), A Text Book of EconomicBotany. Wiley, New Delhi, India, p. 629.

3.       Nikhat F,D.Satyanarayana,Arun B Joshi, Phytochemical and pharmacological investigation on roots of Syzygium cuminii (L) Skeel  Asian Journal of Research in Chemistry. 2008; 2(2): 218-221.

4.       O’Neil MJ.Ed.The Merk index- an encyclopedia of chemical, Drug and biological.14th edn. Merk and co inc 2006,1921




Received on 22.11.2010        Modified on 16.12.2010

Accepted on 30.12.2010        © AJRC All right reserved

Asian J. Research Chem. 4(10): Oct., 2011; Page 1542-1543