A simple Reverse Phase liquid chromatographic(HPLC) method for the determination of Levetiracetam in human plasma has been developed. Separation was achieved on a Prontosil C8 (150 x 4.6 mm, 5 µm) column with mobile phase composition of [pH 3.8 buffer: acetonitrile] (Solution A): HPLC grade water (Solution B) in the ratio 60:40 v/v with ultra violet detection at 210nm. Lower limit of quantitation was 200ng/ml.Maximum between-run precision was 5.91%. Mean extraction recovery was found to be 100.02 to 103.06%. Stability study showed that after three freeze-thaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 48h and at -20° for 2months. Before injecting onto HPLC system, the processed samples were stable for at least 6h.The method was used to perform bioequivalence study in human.
Cite this article:
G.V.H. Raju, S. Ganapathy, D.G. Sankar, P.Y. Naidu. Development and Validation of an HPLC Method for Analysis of Levetiracetam in Human Plasma. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 776-780.