Author(s):
Harishchandra Jirimali, Saiprasad Santre, Vidya Kokne, Bhausaheb More
Email(s):
hdj739@gmail.com , bhaumore1@gmail.com
DOI:
10.52711/0974-4150.2026.00058 
Address:
Harishchandra Jirimali1*, Saiprasad Santre1,2, Vidya Kokne1,2, Bhausaheb More1*
1Regional Forensic Science Laboratory, Nanded (MS) India- 431601.
2Department of Chemistry, Yeshwant Mahavidyalaya, Nanded (MS) India- 431602.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
A chromogenic spray reagent based on Phosphomolybdic Acid (PMA) is introduced for the selective detection and identification of Monocrotophos, a widely used organophosphate insecticide, through Thin Layer Chromatography (TLC). The reagent, easily prepared by simply dissolving PMA in ethanol. The PMA forms a distinct light purple-pink chromogenic adduct with Monocrotophos. The method demonstrates high selectivity in distinguishing Monocrotophos even in the presence of other organophosphorous insecticides and biological interferences, those found in viscera. The assumption is that the color formation is attributed to a redox mechanism involving the reduction of Mo (VI) to Mo(V). This approach is simple, sensitive, and suitable for forensic toxicological analysis of Monochrotophos in biological and forensic samples.
Cite this article:
Harishchandra Jirimali, Saiprasad Santre, Vidya Kokne, Bhausaheb More. Use of Phosphomolybdic Acid as a Chromogenic Reagent for the Selective Detection of Monocrotophos in Biological Samples. Asian Journal of Research in Chemistry.2026; 19(4):391-4. doi: 10.52711/0974-4150.2026.00058
Cite(Electronic):
Harishchandra Jirimali, Saiprasad Santre, Vidya Kokne, Bhausaheb More. Use of Phosphomolybdic Acid as a Chromogenic Reagent for the Selective Detection of Monocrotophos in Biological Samples. Asian Journal of Research in Chemistry.2026; 19(4):391-4. doi: 10.52711/0974-4150.2026.00058 Available on: https://ajrconline.org/AbstractView.aspx?PID=2026-19-4-15
7. REFERENCES:
1. C.P. Harshitha, H.K. Narendra Kumar, N. Chandra Mohana, S. Satish. Biodegradation of monocrotophos by Stenotrophomonas maltophilia and its potential in vitro plant growth promoting activities. The Microbe. 2024; 4:100101 doi.org/10.1016/j.
2. Pawar U. D., Pawar C.D., Kulkarni U.K., Pardeshi R.K., Farooqui M., Shinde D.B. New Chromogenic Reagent for High-Performance Thin-Layer Chromatographic Detection of Organophosphorus Insecticide Monocrotophos in Biological Materials. J. of Planar Chrom. 2019; 32(1): 61-64 doi: 10.1556/1006.2019.32.10
3. Pawar U. D., Pawar C.D., Dhobal B.S., Pardeshi R.K. New Chromogenic Spray Reagent for TLC Detection and Identification of Organophosphorus Insecticide Monocrotophos in Biological Material. To Chem. Journal. 2019; 6.
4. Gurusamy K. and Thangadurai S. Separation and Detection of Monocrotophos by Chromatography Methods in Forensic Samples. J. of Forensic Sci. and Crim. 2019; 7(3).
5. Krishna V. Kulkarni, Davanand B. Shinde, Dhananjay V. Mane, and Manchak V. Garad. A New Chromogenic Spray Reagent for Detection and Identification of Monocrotophos. Journal of Planar Chromatography. 2009; 22(2): 133–135 doi: 10.1556/JPC.22.2009.2.10
6. Chandegaonkar V. R. A New Chromogenic Spray Reagent for Detection and Identification of Monocrotophos. J. of Planar Chrom. 2008; 213: 199-200 doi: 10.1556/JPC.21.2008.3.9
7. Patil V. B., Shingare M.S. Thin-Layer Chromatographic Detection of Monocrotophos in Biological Materials. Talanta, 1994; 41(12): 2127-2130
8. Kulkarni K. V., Shinde D.B., Mane D.V., Garad M.V. A new chromogenic spray agent for detection and identification of Monocrotophos. J. of Planar Chrom. 2009; 22(2): 133-135 doi: 10.1556/JPC.22.2009.2.10
9. Jirimali, H., Santre, S., Deonikar, S. et al. A mini review on study of forensically important poisoning cases involving bromadiolone. Forensic Sci Med Pathol. 2025; 21: 971–978. doi.org/10.1007/s12024-025-00991-z
10. Bhaskar T. Bhoir, Harishchandra D. Jirimali, Vijaykumar M. Navale, Shubhangi N. Deonikar, Bhausaheb P. More. Analysis of traces of the transferred paints by X-ray Fluorescence and UV-Vis spectroscopy to identify the suspect's vehicle for the Forensic science purpose: A Case Study. Asian Journal of Research in Chemistry. 2024; 17(5): 285-8. doi: 10.52711/0974-4150.2024.00049
11. Harishchandra Jirimali, Saiprasad Santre, Shubhangi Deonikar, Bhausaheb More, Sanjay Mhaske. A New Chromogenic Spray Reagent for Detection and Identification of Bromadiolone in Biological Samples. Asian Journal of Research in Chemistry. 2026; 19(2): 109-2. doi: 10.52711/0974-4150.2026.00018
12. Subhasish Chaudhur, Biswajit Chandra Das. Detection of Commonly used Carbamates Thin Layer Chromatography and Spot Test Methods Asian J. Research Chem. 2012; 5(11): 1365-1367
13. Babu Ganesan, Perumal Perumal, Vijaya Baskaran Manickam, Surya Rao Srikakolapu, Srujana Divya Gotteti, Latha Sundaresan Thirumurthy. Simultaneous Determination of Strychnine and Brucine in Herbal Formulation by RP-HPLC. Asian J. Research Chem. 2010; 3(3): 720-723.
14. N.V.S. Venugopal, B. Sumalatha. UV-Spectrophotometric Determination of Acephate and Profenofos (organophosphate) Pesticides in Buffer Medium. Asian J. Research Chem. 2012; 5(4): 526-528.
15. S. D. Garway, D. G. Garway, Y. T. Gaikwad, R.M.R. Azad, G.H. Pandya. Study of Quality Control and Uncertainty in Multiresidue Pesticide Estimation in Packaged Drinking Water Using GC- MS/MS. Asian J. Research Chem. 2012; 5(4): 462-468.
16. M. Aruna, K. Kavitha, R. Madhusudhan Raju, K. Narasimha Reddy. Analysis of multi chemical class pesticide residues in Citrus by LC-MS/MS adopting QuAChERS technique. Asian J. Research Chem. 2016; 9(6): 260-270. doi: 10.5958/0974-4150.2016.00043.2
17. Mahesh K. Saini, Sudeep Mishra, Samsul Alam, Lalitesh K. Thakur, Ompal Singh, Syed K. Raza. Method Development and Validation of Multiclass Pesticide Residues and Metabolites in Wheat by GC-ECD and GC-MS. Asian J. Research Chem. 2016; 9(1): 13-21. doi: 10.5958/0974-4150.2016.00003.1
18. S. D. Garway, D. G. Garway, Y. T. Gaikwad, R.M.R. Azad, G.H. Pandya. Study of Quality Control and Uncertainty in Multiresidue Pesticide Estimation in Packaged Drinking Water Using GC- MS/MS. Asian J. Research Chem. 2012; 5(4): 462-468. doi:
19. Mukund Nagarnaik, Arun Sarjoshi, Girish Pandya. Assessment of QC and Uncertainty in Multiresidue Pesticides analysis in water with GC- ECD/NPD. Asian J. Research Chem. 2014; 7(3): 304-309.
20. Botsa. Parvatamma, Tentu. Nageswara Rao. A New Validated HPLC method for determination of Cyantraniliprole and its metabolite residues in tomato fruit. Asian J. Research Chem. 2015; 8(6): 383-388. doi: 10.5958/0974-4150.2015.00064.4
21. Entu Nageswara Rao, Botsa Parvatamma, Karri Apparao, Jakka Mastan. Evaluation of Residues of Chlorpyrifos and its metabolite in green apples. Asian J. Research Chem. 2017; 10(3): 349-353. doi: 10.5958/0974-4150.2017.00059.1