ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): A. Gupta, S. K. Das, A. K. Banik

Email(s): amritagupta1982@gmail.com

DOI: Not Available

Address: A. Gupta*, S. K. Das and A. K. Banik
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
*Corresponding Author

Published In:   Volume - 6,      Issue - 7,     Year - 2013


ABSTRACT:
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB200 developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl2 in synthetic medium, 0.2 (M) CaCl2 for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.


Cite this article:
A. Gupta, S. K. Das, A. K. Banik. Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200. Asian J. Research Chem. 6(7): July 2013; Page 613-617.


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Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

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