ABSTRACT:
DOPA is a precursor of dopamine and acts as a neuro transmitter. Oxidation of it to dopaquinone is a fast reaction by o-diphenol oxidase. Discrepancies in this reaction are responsible for diverse type of disease and disorders. o-diphenol oxidase was purified on natural column and immobilized on agar- Abelmoschus esculentus polymer. Its immobilized o-diphenol oxidase activity showed Vmax =167µmol/lit/min, Km= 2X10-3, Optimum PH = 6.8, Optimum temperature 800C, Ea = 160.45Kcal/mol and observed highest activity at 45min. behavior of encapsulated activity was compared with free o-diphenol oxidase.
Cite this article:
Anita S. Goswami Giri. Kinetics of o-diphenol oxidase Immobilized on Agar- Abelmoschus esculentus Polymer. Asian J. Research Chem. 7(11): November, 2014; Page 119-924.
Cite(Electronic):
Anita S. Goswami Giri. Kinetics of o-diphenol oxidase Immobilized on Agar- Abelmoschus esculentus Polymer. Asian J. Research Chem. 7(11): November, 2014; Page 119-924. Available on: https://ajrconline.org/AbstractView.aspx?PID=2014-7-11-5