Author(s):
Md. Al Imran Hossain, Mst. Jesmin Aktar, Mehedi Hasan Naim, Lutfun Nahar Chowdhury, Mohua Islam, Md. Saifur Rahman, Md. Ekhlass Uddin, Md. Saddam Hossain, Md. Tariqul Islam, Md. Ali Asraf, Md. Faruk Hossen, Md. Kudrat-E-Zahan
Email(s):
kudrat.chem@ru.ac.bd
DOI:
10.52711/0974-4150.2026.00061 
Address:
Md. Al Imran Hossain1, Mst. Jesmin Aktar2, Mehedi Hasan Naim3, Lutfun Nahar Chowdhury1, Mohua Islam3, Md. Saifur Rahman4, Md. Ekhlass Uddin5*, Md. Saddam Hossain5, Md. Tariqul Islam,5 Md. Ali Asraf5, Md. Faruk Hossen5, Md. Kudrat-E-Zahan5*
1Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh.
2Department of Pharmacy, Daffodil International University, Dhaka, Bangladesh.
3Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.
4Department of Botany, University of Rajshahi, Rajshahi, Bangladesh.
5Department of Chemistry, University of Rajshahi, Rajshahi, Bangladesh.
*Corresponding Author
Published In:
Volume - 19,
Issue - 5,
Year - 2026
ABSTRACT:
This study reports the development of a new stability-indicating reverse-phase high-pressure liquid chromatography (RP-HPLC) assay method to quantify sodium hyaluronate in different types of pharmaceutical dosage forms and various cosmetic products. The technique used is isocratic elution on both Waters Xbridge and Kromasil C18 column (250 × 4.6 mm, 5 µm particle size) with a mobile phase containing buffer at pH 4.5 (Sodium dihydrogen phosphate 0.01 M solution) and methanol (85:15 v/v). UV detection at 205 nm was used to monitor the analyte. However, Waters Xbridge C18 column (250 × 4.6 mm, 5 µm particle size) was finally found suitable for Sodium hyaluronate. Sodium hyaluronate showed linearity over the concentration range of 7.5–60 µg/ml and a retention time of roughly 2.90 minutes. The Limit of Detection (LOD) and Limit of quantification (LOQ) were 0.15 and 0.30 µg/ml respectively. The ICH (International Council for Harmonisation) recommendations were followed in determining the analytical performance parameters. The method was simple, precise, accurate, robust, and rapid, making it suitable for quantifying sodium hyaluronate in different types of pharmaceutical products and cosmetics. This analytical method is specialized for quantification of Sodium hyaluronate present in various formula of pharmaceuticals and cosmetics which made it unique from other analytical method.
Cite this article:
Md. Al Imran Hossain, Mst. Jesmin Aktar, Mehedi Hasan Naim, Lutfun Nahar Chowdhury, Mohua Islam, Md. Saifur Rahman, Md. Ekhlass Uddin, Md. Saddam Hossain, Md. Tariqul Islam, Md. Ali Asraf, Md. Faruk Hossen, Md. Kudrat-E-Zahan. Validated Stability-Indicating RP-HPLC Method for Quantitative Determination of Sodium Hyaluronate in Pharmaceuticals and Cosmetic Formulations. Asian Journal of Research in Chemistry. 2026; 19(5):412-4. doi: 10.52711/0974-4150.2026.00061
Cite(Electronic):
Md. Al Imran Hossain, Mst. Jesmin Aktar, Mehedi Hasan Naim, Lutfun Nahar Chowdhury, Mohua Islam, Md. Saifur Rahman, Md. Ekhlass Uddin, Md. Saddam Hossain, Md. Tariqul Islam, Md. Ali Asraf, Md. Faruk Hossen, Md. Kudrat-E-Zahan. Validated Stability-Indicating RP-HPLC Method for Quantitative Determination of Sodium Hyaluronate in Pharmaceuticals and Cosmetic Formulations. Asian Journal of Research in Chemistry. 2026; 19(5):412-4. doi: 10.52711/0974-4150.2026.00061 Available on: https://ajrconline.org/AbstractView.aspx?PID=2026-19-5-2
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