ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Neela Manish Bhatia, Snehal Jawaharlal Deshmane, Harinath Nivrutti More, Prafulla Balkrishna Choudhari

Email(s): neela.bhatia08@rediffmail.com

DOI: Not Available

Address: Neela Manish Bhatia, Snehal Jawaharlal Deshmane, Harinath Nivrutti More, Prafulla Balkrishna Choudhari
Bharati Vidyapeeth college of Pharmacy, Near Chitranagari , Morewadi , Kolhapur, Maharashtra, 416013, India

Published In:   Volume - 2,      Issue - 4,     Year - 2009


ABSTRACT:
Two new simple, selective economical and reproducible UV spectrophotometeric method simultaneous estimation of two component drug mixture of Amlodipine besylate (AB)) and hydrochlorothiazide (HCT) in pharmaceutical preparation, blood sample and urine has been developed. The first method depends on first derivative method at 245 and 238 nm for AB and HCT also in plasma and urine respectively. The linearity over the concentration ranges 2.5-17.5µg/ml for both the drugs AB and HCT. The second method is based on two wavelength method, which uses the difference of absorbance value at 271 nm and 395 nm for estimation of Hydrochlorothiazide (HCT) and absorbance at 363 nm for amlodipine besylate (AB). In formulation, plasma and urine Methanol: water (50:50v/v) was used as solvent, in which Amlodipine besylate and Hydrochlorothiazide shows linearity in the range of 2.5-17.5 µg/ml for both the drugs respectively. Standard deviation was <1.5 in the assay of tablets. This method validated and the result was compared statically. The %RSD value was satisfactorily low and recovery was close to 100% indicating reproducibility and accuracy of this method. Both the drug obeys Beer’s law in the concentration range employed for analysis. Full analytical validation of this method performed according to International Conference on Harmonization Q2 (R1) and USFDA Bioanalytical methods guidelines. These proposed methods were found to be simple, accurate, rapid, specific, economic and advantageous in having lower limit of detection.


Cite this article:
Neela Manish Bhatia, Snehal Jawaharlal Deshmane, Harinath Nivrutti More, Prafulla Balkrishna Choudhari. Simultaneous Spectrophotometric Estimation of the Amlodipine Besylate and Hydrochlorothiazide in Pharmaceutical Preparations and Biological Samples. Asian J. Research Chem. 2(4):Oct.-Dec. 2009 page 393-397.


Recomonded Articles:

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Banerjee S, Bonde CG, Merukar SS, Patil YR

DOI:         Access: Open Access Read More

Author(s): Ismail, R Rajavel, M Ganesh, M Jagadeeswaran, K Srinivasan, J Valarmathi, T Sivakumar

DOI:         Access: Open Access Read More

Author(s): Manorama B. Motegaonkar, S. D. Salunke

DOI: 10.5958/0974-4150.2017.00025.6         Access: Open Access Read More

Author(s): PA Jadhav, CS Raut, JP Bidada, BB Buwa, PN Dhabale, SC Dhawale

DOI:         Access: Open Access Read More

Author(s): S. Ganapathy, G.V.H. Raju, D.G. Sankar, Pettla Y. Naidu

DOI:         Access: Open Access Read More

Author(s): Sachin R Shinde , Suvarna I Bhoir, Namdev S Pawar, Suman B Yadav, Ajay S Ghumatkar, Ashok M Bhagwat

DOI:         Access: Open Access Read More

Author(s): J Vanitha, A Saravana Kumar, M Ganesh, VS Saravanan

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags