ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Arvind B Karadi Kalpana Jyothi M, Shiva Kumar CH, Appala Raju S., Shobha Manjunath, Ashok L. Ganure

Email(s): arvindabk@rediffmail.com

DOI: Not Available

Address: Arvind B Karadi1* Kalpana Jyothi M1, Shiva Kumar CH1, Appala Raju S.1, Shobha Manjunath1 and Ashok L. Ganure2
1Matoshree Tarabai Rampure Institute of Pharmaceutical Sciences, Gulbarga-585 105 (K.S.) India
2K.J. College of Pharmacy, Vadasma (North Gujarat), India
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
A Simple and precise reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the quantitative determination of Diacerein in bulk and its pharmaceutical formulations. Chromatographic separation was performed by using column XDB C8 (4.6x 150 mm, 5µm) with a mobile phase comprising of a mixture of phosphate buffer and Acetonitrile (50:50,v/v) and pH adjusted to 3 with orthophosphoric acid, at a flow rate of 0.8 ml/min with detection at 254nm. Separation was completed in less than 10 min. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, ruggedness and robustness. Linearity of Diacerein was found to be in the range of 10-100µg/ml and correlation coefficient was found to be 0.999. The retention time of Diacerein was 3.3 min; the separation was performed at an ambient temperature. The limit of Detection and limit of quantitation for Diacerein was found to be 3.6 and 9.95 (S/N ratio). There was no significant difference in the intraday and inter day analysis of Diacerein determined for five different concentrations using this method. The RSD value 0.3% indicates a high precision of the analytical method. The proposed method was simple, sensitive, precise, accurate and useful for routine quality control analysis.


Keywords:

Cite this article:
Arvind B Karadi Kalpana Jyothi M, Shiva Kumar CH, Appala Raju S., Shobha Manjunath, Ashok L. Ganure. Determination of Diacerein by a Simple Isocratic RP-HPLC Method. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 581-584.


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DOI: 10.5958/0974-4150 

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