K. Vijaya Sri, S. Sruthi, l.D. Srinivas
K. Vijaya Sri*, S. Sruthi and l.D. Srinivas
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy,
Maisammaguda, Secunderabad-500 014, Andhra Pradesh, India
Volume - 7,
Issue - 9,
Year - 2014
The main objective was to develop and validate the UV-spectrophotometric method for the estimation of tolvaptan in bulk and pharmaceutical formulations as per ICH guidelines. The initial stock solution of Tolvaptan was prepared in acetonitrile. The ?max of tolvaptan was found to be 267 nm it was proved linearity in the concentration range 1–10 µg/ml with a correlation coefficient value of 0.999. The accuracy studies of proposed method was performed at three different levels, i.e., 50%, 100%, and 150% and recovery was found to be in the range of 100.4%.The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.34 and 0.94 µg/ml, respectively.The % RSD less than 2 which indicates the accuracy and precise of the method. The above method was a rapid tool for routine analysis of tolvaptan in the bulk and in the pharmaceutical dosage form.
Cite this article:
K. Vijaya Sri, S. Sruthi, l.D. Srinivas. UV Spectrophotometric Method for the Estimation of Tolvaptan in Bulk and Pharmaceutical Formulations. Asian J. Research Chem. 7(9): September 2014; Page 773-776.